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KMID : 0380219850180040410
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Journal of Biochemistry and Molecular Biology
1985 Volume.18 No. 4 p.410 ~ p.416
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Solid-Phase Synthesis of the human insulin B (5-9) Analog with Special Rotary Reactor
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Kook Soon-Uoong
Hong Nam-Joo
Shin Seung-Dong
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Abstract
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A simple, inexpensive apparatus has been designed to perform the solid-phase peptide synthesis. It consists of 60 ㎖ capacity glass cylinder (5×6 ㎝) fritted filter disc embeded in the flask and a one-way 1.5 ㎜ stopcock. The rotary reactor was used to avoid splashing any resin particles up on the sides of the vessel. This peptide synthesizer proved to be both reliable and convenient dining synthesis of the human insulin B (5-9) analog as a model compound. All coupling steps were monitored by ninhydrin procedure. The siolated peptide mixture resulting from Beyerman cleavage (Beyerman, 1979) was analyzed by HPLC and $gt;98% purity of the total product was shown to be the desired products.
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